Gene Manipulation: Unlocking Genetic Potential With Gene Pumping
Gene pumping utilizes enzymes (polymerase, ligase) to isolate, amplify, and modify genes. It involves gene cloning via restriction digestion, ligation, and PCR. The Green Fluorescent Protein (GFP) and luciferase allow visualization and measurement of gene activity. Recombinant DNA combines genes from different sources for genetic manipulation. Moreover, gene pumping techniques, such as transfection and transformation,…