Biotinylated Secondary Antibodies: Amplify Detection Sensitivity In Immunological Techniques
Biotinylated secondary antibodies are antibodies that have been chemically conjugated to biotin, a small molecule with a high affinity for streptavidin and avidin. This conjugation allows for the binding of multiple streptavidin- or avidin-conjugated detection reagents, resulting in signal amplification and increased sensitivity in immunohistochemistry, Western blotting, and flow cytometry. By employing biotinylated secondary antibodies,…