Crystal Violet Stain: A Key Technique For Gram Staining And Bacterial Differentiation
The crystal violet stain, an integral part of the Gram’s staining protocol, differentiates between Gram-positive and Gram-negative bacteria. Composed of crystal violet, Gram’s stain involves binding the stain to bacterial cell walls. Gram-positive bacteria, with their thick peptidoglycan layers, retain the stain and appear purple, while Gram-negative bacteria, lacking the thick peptidoglycan, lose the stain…